AEG Electrolux COMPETENCE 5133 V User Manual Page 27

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Tit l
Id tifi i
d
Tit
e
l
:
Id
en
tifi
z
i
erung un
d
Charakterisierun
g
von Leukämie-
g
spezifischen T-Zellen (PM30)
Projektpartner:
III
Medizinische
Klinik
III
.
Medizinische
Klinik
,
Prof. Dr. med. Angela Krackhardt und
Kiadis
Pharma
Kiadis
Pharma
www.m4.de
Aims
Identification/Isolation of T cells in ATIR™ responsible for graft versus
leukemia effect
leukemia
effect
ATIR/post-transplant donor T cells
HLA monomer
Leukemiapeptide
Co-incubation with peptide
pulsed recipient LCL or HLA-
tr
a
n
sge
ni
c
ce
ll lin
es
HLA
monomer
production
AG Busch, TUM
HLA monomer
Fluorochrome
asge c
ce
es
IFN-γ
capture
ELISpot
Fluorochrome
www.m4.de
Results: ATIR™
p
hase I/II clinical trial
p
donor
ATIR: Personalized T-cell product, depleted of allo-reactive T cells
Leukoc
y
te collection
Activation
Personalized
T
ll
patient
TH942
Allo-reactive
Light
Activation of
y
of allo-
reactive
T-cells in
one-way
MLR
T
-ce
ll
product
ATIR
Infusion of
ATIR four
weeks after
haploidentical
Gamma
irradiation
patient
T-cells
incorporate
novel
compound
TH9402
TH9402 by
light results
in depletion
of allo-
reactive
T-cells
Transplant related mortality
Overall Survival
haploidentical
HSCT
Transplant
related
mortality
Overall
Survival
ATIR:
60% patients active disease
at HSCT
Naked haplo:
All patients in remission
at HSCT
at
HSCT
www.m4.de
Results: Reactivit
y
of ATIR™
p
roduct
yp
donor
Recipient
alloreactive
Other
T-cells
3rd party
alloreactive
ATIR
Donor
Viral
reactive
?
ATIR
Leukemia
reactive
?
www.m4.de
24
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